Custom-Mixed Genotyping Panels, Genotyping Panels, Signet Customized Genotyping Panels

Individual assays in the Signet™ Genotyping Panels can be configured into custom-mixed Genotyping Panels. The Signet™ Genotyping Panels are specific multiplexes that detect polymorphisms and gene variants associated with a variety of health and nutritional conditions including heart and bone health, insulin sensitivity, inflammation, obesity, Alzheimer’s disease, blood disorders and oncology. These assays have applications in nutrigenomics, pharmacogenomics, and toxicogenomics. In addition, we offer lifestyle panels that can test for athletic ability and for the predisposition to male baldness. Download the full list of panels.

Flexibility is a key feature - for example, ten SNPs from five different panels can be reconfigured into a single customized panel. To configure your custom panel, choose as many assays as you need from the full assay list and send the list to Marligen for a custom quotation. Once you have chosen your SNPs or variants, Marligen scientists will reconfigure the panels to your specifications, manufacture reagents, validate performance, and send you a complete package ready for use.

To request a quote or additional information for customized Genotyping Panels please fill out a quote request.

Advantages:

High-throughput format allows hundreds of samples to be analyzed in one day
Excellent allelic discrimination yields >99% accuracy
Standardized assay methodology and filter-plate format maximizes reproducibility
Cost effective solution for detecting multiple genetic variants in one sample

Technology and Protocol

Marligen’s genotyping panels use the primer extension assay method:

Signet™ Genotyping Technology Hybridization Method

Protocol for primer extension (print chart)
Sample data from primer extension assay (ex. 1) (ex. 2) (print charts)
Example package insert

Components supplied with custom kit

Taq DNA Polymerase
2X PCR Mix
Exonuclease I /Shrimp Alkaline Phosphatase
2X Primer Extension Mix
Bead Mix
Hybridization Buffer
Detection Reagent
Filter Plates (if required)
9948 Control DNA
9947A Control DNA
Protocol
Template for data acquisition and data analysis software

Required but not supplied

Platinum Tsp genotyping (Invitrogen)
PCR Plate or tubes
Filter plate manifold with vacuum source

All of Marligen's Signet™ Genotyping Assays are configured to be used in conjunction with the Luminex™ xMAP System for multiplexed analysis. For researchers interested in trying out our assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services.

Please inquire for adaptation for use on other platforms.

Signet™ Custom-Mixed Genotyping Panels