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DNA Mass Ladders

technical notes faqs

Marligen’s DNA Mass Ladder are designed for accurate estimation of the size and quantity of double-stranded DNA fragments following electrophoresis. The DNA Mass Ladders are conveniently supplied in loading buffer for direct application to either TAE or TBE agarose gels.

Broad Range DNA Mass Ladder

pcr dna mass ladder

Figure 1 demonstrates the performance of the Broad Range DNA Mass Ladder. The lane was loaded with 10 μL of the Broad Range DNA Mass Ladder run on a 1% agarose gel in 1X TAE and stained with ethidium bromide. Sharp, well-spaced bands covering a broad range of fragment sizes from 300 bp up to 10 kb make determining both the molecular weights and amounts of double-stranded DNA fragments simple and fast.

PCR DNA Mass Ladder

broad range dna mass ladder

Figure 2 demonstrates the performance of the PCR Mass Ladder. The lane was loaded with 10 μL of the PCR DNA Mass Ladder run on a 1.2% agarose gel in 1X TAE and stained with ethidium bromine. Sharp, well-spaced bands covering a broad range of fragment sizes from 100 bp up to 2 kb make determining both the molecular weights and amounts of double-stranded PCR products simple and fast. A higher intensity reference band runs at 500bp.