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Marligen offers a variety of cost effective DNA modifying and cloning enzymes
that complement our large portfolio of DNA purification products including:
T4 DNA Ligase
T4 DNA Ligase catalyzes the formation
of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl
termini in duplex DNA or RNA. This enzyme will join blunt and cohesive
end termini as well as repair single- stranded nicks in duplex DNA, RNA
or DNA/RNA hybrids.
Advantages:
- Cost Effective
- Offered in two concentrations: 400 U/µL and 2,000 U/µL
- Conveniently supplied with 10X T4 DNA
Ligase Reaction Buffer
[Note Marligen’s T4 DNA Ligase products are supplied with Cohesive
End Ligation Units as the unit of measure, For comparison with similar
products 67 Cohesive End Ligation Units is approximately equivalent to
One Weiss Unit.]
Request
a Sample of T4 DNA Ligase >>
Rapid Ligation Kit
The Rapid Ligation
Kit is a combination of T4 DNA Ligase and an optimized reaction buffer
designed to ligate blunt or cohesive end DNA fragments in 5 minutes at
room temperature.
Advantages:
- Fast ligation at room temperature
- Suitable for all common ligation reactions
- Cost Effective
- Conveniently supplied with 2X Rapid Ligation
Buffer
Request
a Sample of the Rapid Ligation Kit >>
Uracil DNA Glycosylase (UDG)
Uracil DNA Glycosylase
(UDG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil
and sugar, leaving an abasic site in uracil-containing single or double-stranded
DNA. The enzyme shows no measurable activity on short oligonucleotides
(<6 bases), or RNA substrates.
Advantages:
- Cost Effective
- Conveniently supplied with 10X Reaction Buffer
Order DNA Modifying and Cloning Enzymes
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