M-MuLV Reverse Transcriptase

Product Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template. M-MuLV Reverse Transcriptase is completely deficient in 3’-5’ proofreading exonuclease function.

Applications
cDNA synthesis from single-stranded RNA or DNA.

Multiple transcripts can be analyzed from a single RT-reaction.

Source of Protein
A recombinant E. coli strain carrying the Moloney Murine Leukemia Virus Reverse Transcriptase gene.

Components
1. M-MuLV Reverse Transcriptase supplied in 10 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% NP40 alternative, 50% Glycerol.

2. 10X Reaction Buffer containing 500 mM Tris-HCl, pH 8.3, 30 mM MgCl₂, 750 mM KCl, 100 mM DTT, 1 mM EDTA.

Storage Conditions
Store at –20°C.

Concentration
200 units/µL

Unit Definition
1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Quality Assurance
Purified free of contaminating endonucleases and exonucleases. In addition, enzyme purity is analyzed by SDS-PAGE, and negligible E.coli genomic DNA is confirmed by qPCR.

Suggested Reaction Conditions
Incubate at 37°C with 1X Reaction Buffer supplemented with 100 μM dNTPs (not included).