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Transcription Factor Microplate Assays technical notes faqs

 
 
Marligen’s Chemiluminescent Transcription Factor Microplate Assays allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells and are designed to be used in combination with Marligen’s Nuclear Extraction Kit to ensure optimal performance.
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Advantages:

  • Up to 100-fold more sensitive than gelshift assays
  • Eliminates radioactivity and the use of gels
  • Superior sensitivity minimizes amount of nuclear extracts required
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Assays with multiple species including humans, monkeys and rodents
  • Direct quantitation of binding activity without the need for antibodies.

Currently, Marligen offers 11 different assays in multiplex format. These assays include AP-1, CRE-ATF, CREB, EGR, GATA, MEF2, Myc-Max, NF-1, NFκB, Octamer, and PPAR. Click here to obtain more information on the sites used for these assays.

Order Transcription Factor Microplate Assays

  Catalog No. Item Name Size U.S. List Price (US$)
11942-096
Pkg Insert
AP-1 Transcription Factor Microplate Assay 96 rxns 530.00
11945-096
Pkg Insert
NFκB Transcription Factor Microplate Assay 96 rxns 530.00
11946-096
Pkg Insert
EGR Transcription Factor Microplate Assay 96 rxns 530.00
11947-096
Pkg Insert
NF-1 Transcription Factor Microplate Assay 96 rxns 530.00
11953-096
Pkg Insert
PPAR Transcription Factor Microplate Assay 96 rxns 530.00
11978-096
Pkg Iinsert
Myc-Max Transcription Factor Microplate Assay 96 rxns 530.00
11979-096
Pkg Insert
CREB Transcription Factor Microplate Assay 96 rxns 530.00
11980-096
Pkg Insert
GATA Transcription Factor Microplate Assay 96 rxns 530.00
11981-096
Pkg Insert
Octamer Transcription Factor Microplate Assay 96 rxns 530.00
11982-096
Pkg Insert
MEF-2 Transcription Factor Microplate Assay 96 rxns 530.00
11983-096
Pkg Insert
CRE-ATF Transcription Factor Microplate Assay 96 rxns 530.00
11906-100
Pkg Insert
Nuclear Extraction Kit 100 rxns 135.00
11941-100
Pkg Insert
Whole Cell Extraction Reagent 100 rxns 135.00
60319 10X Wash Buffer for Transcription Factor Microplate Assays 100 mL 47.00
11938-001
Pkg Insert
HL-60 Nuclear Extract Controls (treated and untreated) 2 x 100 μg 210.00
11934-001
Pkg Insert
Protein Control (NF-1/NFkB) 20 μL 82.00

U.S. Pricing Only. For international pricing and orders please contact one of our international distributors.

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