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Transcription Factor Microplate Assays technical notes faqs
 
Marligen’s Chemiluminescent Transcription Factor Microplate Assays allow researchers to quantitate the DNA binding activity of transcription factors in nuclear extracts prepared from cultured cells and are designed to be used in combination with Marligen’s Nuclear Extraction Kit to ensure optimal performance.
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Advantages:

  • Up to 100-fold more sensitive than gelshift assays
  • Eliminates radioactivity and the use of gels
  • Superior sensitivity minimizes amount of nuclear extracts required
  • Results in less than four hours
  • Compatible with standard plate luminometers
  • Assays with multiple species including humans, monkeys and rodents
  • Direct quantitation of binding activity without the need for antibodies.

Currently, Marligen offers 11 different assays in multiplex format. These assays include AP-1, CRE-ATF, CREB, EGR, GATA, MEF2, Myc-Max, NF-1, NFκB, Octamer, and PPAR. Click here to obtain more information on the sites used for these assays.

Order Transcription Factor Microplate Assays

  Catalog No. Item Name Size U.S. List Price (US$)
PM100006
Pkg Insert
Adherent Cell Lysis Solution (11984-050) 50 mL $59
PM100004
Pkg Insert
HL-60 Nuclear Extract Controls (treated and untreated) (11938-001) 2 x 100 µg $216
PM100001
Pkg Insert
Nuclear Extraction Kit (11906-100) 100 rxns $139
PM100005
Pkg Insert
Whole Cell Extraction Reagent (11941-100) 100 rxns $139
PM100002 10X Wash Buffer for Transcription Factor Microplate Assays (60319) 100 mL $48
PM100003
Pkg Insert
Protein Control (NF-1/NFkB) (11934-001) 20 mL $85

To order these products, or contact technical services, please visit www.origene.com/about/contact_us/
For international pricing and orders please contact one of our international distributors.

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