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Multiplex Transcription Factor Assays

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Marligen’s Multiplex Transcription Factor Assays employ a powerful multiplexing approach for simultaneously measuring the binding of many different transcription factors to their Multiplex Transcription Factor Profiling Kitunique regulatory DNA elements. The technology dramatically accelerates studies ranging from basic research in signal transduction and proteomics to target validation, compound screening, toxicity testing and disease characterization.
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Advantages:

  • Speed, ease of use and quantitation vastly outperform EMSAs
  • Excellent sensitivity, specificity, reproducibility and dynamic range versus ELISAs
  • Unparalled speed of data acquisition - ~2,000 results in 3 hours
  • Small sample requirement saves time and money
  • Assays validated in many cells lines, primary cells and tissues from humans, monkeys and rodents
  • Multiple binding sites in assay mimics in vivo binding conditions

Marligen offers 20 different assays that are available in a pre-mixed 20-plex or as single-plexes that can be combined to make your own custom panel. These assays include AP-1, AP2, AR, CRE-ATF, CREB, E2F1-5, E2F6, EGR, GATA, HNF1, HNF4, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD2/3, SP1, and YY1. A Multiplex Transcription Factor Profiling Kit (Cat. No. 11955-096) contains all of the common assay reagents and is required to run a custom multiplex panel. All of these multiplex assays are formatted to be read on the Luminex Reader. Find out more details about the Luminex xMAP® technology and instrumentation.

For researchers interested in trying out our assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services.

Order Multiplex Transcription Factor Assays

  Catalog No. Item Name Transcription Factor Binding Site (s) Size U.S. List Price (US$)
AM100053
Pkg Insert		 Multiplex Transcription Factor Profiling Kit (20-plex) (11954-096) AP-1, AP2, AR, CRE-ATF, CREB, E2F1-5, E2F6, EGR, GATA, HNF1, HNF4, ISRE, Myc-Max, NF-1, NF?B, p53, PPAR, SMAD2/3, SP1, YY1 96 rxns $2986
AM100054
Pkg Insert		 Multiplex Transcription Factor Profiling Kit (11955-096) Common reagents for running multiplex assay kit 96 rxns $246
AM100055
Pkg Insert		 CRE-ATF Site Specific Reagent (11956-096) CRE-ATF 96 rxns $267
AM100056
Pkg Insert		 AP-1 Site Specific Reagent (11957-096) AP-1 96 rxns $267
AM100057
Pkg Insert		 NFkB Site Specific Reagent (11958-096) NFκB 96 rxns $267
AM100058
Pkg Insert		 AR Site Specific Reagent (11959-096) AR 96 rxns $267
AM100059
Pkg Insert		 CREB Site Specific Reagent (11960-096) CREB 96 rxns $267
AM100060
Pkg Insert		 YY1 Site Specific Reagent (11961-096) YY1 96 rxns $267
AM100061
Pkg Insert		 E2F1-5 Site Specific Reagent (11962-096) E2F1-5 96 rxns $267
AM100062
Pkg Insert		 E2F6 Site Specific Reagent (11963-096) E2F6 96 rxns $267
AM100063
Pkg Insert		 SP1 Site Specific Reagent (11964-096) SP1 96 rxns $267
AM100064
Pkg Insert		 Myc-Max Site Specific Reagent (11965-096) Myc-Max 96 rxns $267
AM100065
Pkg Insert		 EGR Site Specific Reagent (11966-096) EGR 96 rxns $267
AM100066
Pkg Insert		 ISRE Site Specific Reagent (11967-096) ISRE 96 rxns $267
AM100067
Pkg Insert		 p53 Site Specific Reagent (11968-096) p53 96 rxns $267
AM100068
Pkg Insert		 GATA Site Specific Reagent (11969-096) GATA 96 rxns $267
AM100069
Pkg Insert		 AP2 Site Specific Reagent (11970-096) AP2 96 rxns $267
AM100070
Pkg Insert		 SMAD 2/3 Site Specific Reagent (11971-096) SMAD 2/3 96 rxns $267
AM100071
Pkg Insert		 HNF1 Site Specific Reagent (11972-096) HNF1 96 rxns $267
AM100072
Pkg Insert		 HNF4 Site Specific Reagent (11973-096) HNF4 96 rxns $267
AM100073
Pkg Insert		 NF-1 Site Specific Reagent (11974-096) NF-1 96 rxns $267
AM100074
Pkg Insert		 PPAR Site Specific Reagent (11975-096) PPAR 96 rxns $267
PM100001
Pkg Insert		 Nuclear Extraction Kit (11906-100) NA 100 rxns $139
PM100005
Pkg Insert		 Whole Cell Extraction Reagent (11941-100) NA 100 rxns $139
PM100003
Pkg Insert		 Protein Control (NF-1/NFkB) (11934-001) NA 20μL $85
AM100086 Multiplex Transcription Factor Testing Service (50-plex) (11907-001) AP-1, AP2, AML-1, AP4, AR, CRE-ATF, C/EBP?, C/EBP?, C/EBP?, CREB, E2F1-5, E2F6, EGR, ER, ETS, GATA, HFH2/3, HIC-1, HIF, HNF-1, HNF-3, HNF-4, HSF-1, ISRE, MEF-2, MTF1, c-Myb, Myc-Max, NFAT, NF-1, NF?B, NFY1, Octamer, p53, PAX6, PBX, PLZF, PPAR, SAF-1, SOX9, STAT, SREBP, SMAD1/5, SMAD 2/3, SP1, SRE, TGIF, TAL1, TCF/LEF and YY1 96 rxns $5750

For international pricing and orders please contact one of our international distributors.

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