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Multiplex Transcription Factor Assays

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Marligen’s Multiplex Transcription Factor Assays employ a powerful multiplexing approach for simultaneously measuring the binding of many different transcription factors to their multiplex transcription factor profiling kitunique regulatory DNA elements. The technology dramatically accelerates studies ranging from basic research in signal transduction and proteomics to target validation, compound screening, toxicity testing and disease characterization.
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Advantages:

  • Speed, ease of use and quantitation vastly outperform EMSAs
  • Excellent sensitivity, specificity, reproducibility and dynamic range versus ELISAs
  • Unparalled speed of data acquisition - ~2,000 results in 3 hours
  • Small sample requirement saves time and money
  • Assays validated in many cells lines, primary cells and tissues from humans, monkeys and rodents
  • Multiple binding sites in assay mimics in vivo binding conditions

Marligen offers 20 different assays that are available in a pre-mixed 20-plex or as single-plexes that can be combined to make your own custom panel. These assays include AP-1, AP2, AR, CRE-ATF, CREB, E2F1-5, E2F6, EGR, GATA, HNF1, HNF4, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD2/3, SP1, and YY1. A Multiplex Transcription Factor Profiling Kit (Cat. No. 11955-096) contains all of the common assay reagents and is required to run a custom multiplex panel. All of these multiplex assays are formatted to be read on the Luminex Reader. Find out more details about the Luminex xMAP® technology and instrumentation.

For researchers interested in trying out our assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services.

Order Multiplex Transcription Factor Assays

  Catalog No. Item Name Transcription Factor Binding Site (s) Size U.S. List Price (US$)
11954-096
Pkg Insert 		Multiplex Transcription Factor Profiling Kit (20-plex) AP-1, AP2, AR, CRE-ATF, CREB, E2F1-5, E2F6, EGR, GATA, HNF1, HNF4, ISRE, Myc-Max, NF-1, NFκB, p53, PPAR, SMAD 2/3, SP1, YY1 96 rxns 2899.00
11955-096
Pkg Insert 		Multiplex Transcription Factor Profiling Kit Common reagents for running multiplex assay kit 96 rxns 239.00
11956-096
Pkg Insert 		CRE-ATF Site Specific Reagent Set CRE-ATF 96 rxns 259.00
11957-096
Pkg Insert 		AP-1 Site Specific Reagent Set AP-1 96 rxns 259.00
11958-096
Pkg Insert 		NFκB Site Specific Reagent Set NFκB 96 rxns 259.00
11959-096
Pkg insert 		AR Site Specific Reagent Set AR 96 rxns 259.00
11960-096
Pkg Insert 		CREB Site Specific Reagent Set CREB 96 rxns 259.00
11961-096
Pkg Insert 		YY1 Site Specific Reagent Set YY1 96 rxns 259.00
11962-096
Pkg Insert 		E2F1-5 Site Specific Reagent Set E2F1-5 96 rxns 259.00
11963-096
Pkg Insert 		E2F6 Site Specific Reagent Set E2F6 96 rxns 259.00
11964-096
Pkg Insert 		SP1 Site Specific Reagent Set SP1 96 rxns 259.00
11965-096
Pkg Insert 		Myc-Max Site Specific Reagent Set Myc-Max 96 rxns 259.00
11966-096
Pkg Insert 		EGR Site Specific Reagent Set EGR 96 rxns 259.00
11967-096
Pkg Insert 		ISRE Site Specific Reagent Set ISRE 96 rxns 259.00
11968-096
Pkg Insert 		p53 Site Specific Reagent Set p53 96 rxns 259.00
11969-096
Pkg Insert 		GATA Site Specific Reagent Set GATA 96 rxns 259.00
11970-096
Pkg Insert 		AP2 Site Specific Reagent Set AP2 96 rxns 259.00
11971-096
Pkg Insert 		SMAD 2/3 Site Specific Reagent Set SMAD 2/3 96 rxns 259.00
11972-096
Pkg Insert 		HNF1 Site Specific Reagent Set HNF1 96 rxns 259.00
11973-096
Pkg Insert 		HNF4 Site Specific Reagent Set HNF4 96 rxns 259.00
11974-096
Pkg Insert 		NF-1 Site Specific Reagent Set NF-1 96 rxns 259.00
11975-096
Pkg Insert 		PPAR Site Specific Reagent Set PPAR 96 rxns 259.00
11906-100
Pkg Insert 		Nuclear Extraction Kit NA 100 rxns 135.00
11941-100
Pkg Insert 		Whole Cell Extraction Reagent NA 100 rxns 135.00
11938-001
Pkg Insert		 HL-60 Nuclear Extract Control (treated and untreated) NA 2x100 μg 210.00
11934-001
Pkg Insert 		Protein Control (NF-1/NFκB) NA 20 μL 82.00

U.S. Pricing Only. For international pricing and orders please contact one of our international distributors.

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