Nuclear Extraction Kit, Whole Cell Extraction Reagent

Marligen’s Nuclear Extraction Kit is optimized to provide gentle extraction of nuclear and cytoplasmic proteins to maintain transcription factor activity with minimal contamination. The Extracts obtained from cells and tissues are compatible with Marligen’s Multiplex Transcription Factor Kits and Transcription Factor Microplate Assays as well as other applications. Marligen’s Whole Cell Extraction Reagent has been specifically formulated to quickly screen cell lines and tissues for changes in transcription factor activity as well as other biological events.

Advantages:

Nuclear Extraction Kit

Provide nuclear extracts with minimal cross contamination
Highly suited for measuring DNA binding activities of transcription factors
Extract may be used for multiple applications including EMSAs, ELISAs, and Western blotting

Whole Cell Extraction Reagent

Cell lysates in less than 1 hour
Highly suited for high through-put screening of transcription factor activities
Lysate may be used for multiple applications including protein phosphorylation studies and Western blotting

Nuclear Extracts have been prepared from cell lines, primary cells and tissues from multiple species including the following:

293, 3T3 C2C12, COS-7, HeLa , Hep G2, Jurkat, K562, Kas MCF-7, MSC34, MV-1(mink), NIH-3T3, PC-12, Raji, THP-1, U2O5 , U937, WI-38 , Primary Fibroblasts, Primary NK cells, Rat heart, and Human Whole Blood. An array of mouse tissues have also been used to make nuclear extracts including brain, liver, heart, lung, skeletal muscle, pancreas, kidney, spleen, bone marrow, and thymus. For a detailed protocol on how to prepare nuclear extracts from whole blood and selected tissues.

Order Nuclear Extraction Kit