PCR and RT-PCR Enzymes

Taq DNA Polymerase
Taq DNA Polymerase is a thermostable processive, 5’-3’ DNA polymerase. The 94 kDa protein possesses an inherent 5’-3’ nick-translation moiety and lacks a 3’-5’ proofreading function.

Advantages:

• Cost effective
• Suitable for a wide variety of PCR assays
• Optimized for use with Marligen’s Signet Genotyping Assays

Marligen’s Taq DNA Polymerase is supplied at a concentration of 5 U/µL with a 10X PCR buffer containing 100 mM Tris-HCl, pH 8.6, 15 mM MgCl₂, 750 mM KCl.

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M-MuLV Reverse Transcriptase
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA-directed DNA polymerase used for synthesizing a complementary DNA strand. Copying is initiated from a primer using either single-stranded RNA or single-stranded DNA template.

Advantages:

• Improved efficiency for full length cDNA synthesis
• High sensitivity
• Cost effective

Marligen’s M-MuLV Reverse Transcriptase is supplied with a 10X reaction buffer containing 500 mM Tris-HCl, pH 8.3, 30 mM MgCl₂, 750 mM KCl, 100 mM DTT, 1 mM EDTA.

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For international pricing and orders please contact one of our international distributors.