Rapid Total RNA Purification Kits

Technology

Marligen’s Total RNA Isolation Kits rely on disrupting cells and tissues in the presence of chaotropes, which also simultaneously inactivate endogenous nucleases, thereby protecting the RNA from degradation. The solubilized RNA is then adsorbed to a silica membrane under conditions that optimize binding of RNA versus contaminants. Following washes to remove remaining contaminants, the RNA is eluted, ready to use in a variety of applications including RT-PCR, microarray analysis, Northern blotting, RNase protection assays or cDNA library construction.

Performance Characteristics

Figure 1 shows that RNA isolated with Marligen’s Rapid RNA Isolation Kits demonstrates high yields of intact RNA. Figure 2 shows that A260/280 ratios, total yield and overall concentration is very reproducible from prep to prep. Each lot of kits is tested by agarose gel electrophoresis and spectrophotometric analysis to verify the purity and integrity of the resulting RNA.

Fig. 1 Three samples containing 10⁷ Hela cells were purified with the Marligen Total RNA Purification System. Gel results demonstrate a high yield of intact RNA and high reproducibility.

Table 1 below shows data from the purification of total RNA from three identical samples of 10⁷ Hela cells shows excellent purity and yield. RNA is eluted at a high concentration and reproducibility is high with coefficients of variation below 10%.