Multiplex SNP Genotyping, Assay Development Services, Custom SNP Genotyping Assays

Signet™ Genotyping Assays are designed to rapidly and accurately detect genetic variations including single nucleotide polymorphisms (SNPs), small insertions and deletions (indels) and whole gene deletions. In addition to the specific Signet™ Genotyping Panels that Marligen offers, our scientists can work with you to design, develop, and manufacture specific Custom SNP Genotyping Assays. Send us your sequences, and we will design and test the multiplex reactions, manufacture all reagents, validate the performance of the assays and deliver you a complete package ready for use.

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Technology and Protocol

Marligen’s genotyping assays use two different technologies:

Each assay type confers specific benefits and advantages:

Hybridization vs primer extension (print chart)
Protocol for hybridization. (print chart)
Sample data from hybridization assay (print chart)
Protocol for primer extension (print chart)
Sample data from primer extension assay (ex. 1) (ex. 2) (print charts)

Primer Extension Assay Method (components supplied with assay kit)	Hybridization Assay Method (components supplied with assay kit)
Taq DNA Polymerase 2X PCR Mix Exonuclease I /Shrimp Alkaline Phosphatase Bead Mix Hybridization Buffer Detection Reagent Filter Plates (if required) 9948 Control DNA 9947A Control DNA Protocol	Hot-start Taq Polymerase 2X PCR Mix Bead Mix Taq Polymerase Labeling Mix Hybridization Buffer Control DNA Protocol
Required but not supplied	Required but not supplied
Platinum Tsp genotyping (Invitrogen) PCR Plate or tubes Filter plate manifold with vacuum source	PCR Plates
A template for data acquisition and data analysis software is supplied with all custom orders.

All of Marligen's Custom Genotyping Assays are configured to be used in conjunction with the Luminex™ xMAP System for multiplexed analysis. Please inquire for adaptation for use on other platforms.

For researchers interested in trying out our multiplex Genotyping assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services.

Multiplex SNP Genotyping Assay Development