Uracil DNA Glycosylase

Product Description
Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

Recommended Applications
Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase.

Source of Protein
A recombinant E.coli strain carrying the Uracil DNA Glycosylase gene from E.coli K-12.

Components
1. Uracil DNA Glycosylase supplied in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% Glycerol.

2. 10X Reaction Buffer containing 20 mM Tris-HCl, pH 8.2, 1 mM DTT, 1 mM EDTA.

Storage Conditions
Store at –20°C.

Concentration
2 units/µL

Unit Definition
One unit is defined as the amount of enzyme that catalyzes the release of 3.60 nmol of uracil in one hour from a double-stranded, 1.1 kb DNA fragment containing tritiated uracil at 37°C in 1X UDG Reaction Buffer.

Quality Assurance
Purified free of contaminating endonucleases and exonucleases. In addition, enzyme purity is analyzed by SDS-PAGE, and negligible E.coli genomic DNA is confirmed by qPCR.

Suggested Reaction Conditions
Incubate at 37°C with 1X UDG Reaction Buffer.