Vantage™ microRNA Analysis Products

Marligen’s Vantage™ products offer researchers a comprehensive solution for microRNA analysis. Total or low molecular weight RNAs can be isolated using our simple, rapid purification methods. The Vantage™ microRNA Labeling Kit directly labels total or enriched samples with multiple biotins to give the utmost sensitivity in profiling microRNA expression. Our disease-specific Multiplex Detection Panels are formatted on the Luminex xMAP® platform to rapidly and accurately profile the expression levels of microRNAs. The full process from purification to detection can be completed in less than 4 hours and more than a hundred samples can be processed in just one day. View full Vantage™ microRNA analysis process

• Vantage™ RNA Purification Kits
• Vantage™ microRNA Labeling Kits
• Vantage™ microRNA Multiplex Detection Panels
• Vantage™ Custom-Mixed microRNA Detection Panels

The Vantage™ Total RNA and microRNA Purification Kits provide rapid and effective methods of isolating RNAs without the use of phenol or chloroform. Our Total RNA kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA (siRNA). The microRNA Purification Kit specifically isolates and purifies low molecular weight (LMW) RNAs that include the regulatory RNAs such as microRNA and siRNA. The purified LMW RNAs can be used in various downstream application including gene regulation and functional analysis studies.

The Vantage™ microRNA Labeling Kit utilizes a proprietary labeling technology to provide exceptional sensitivity for the detection of endogenous microRNAs. This method can label any RNA sample including total RNA, enriched LMW RNA, amplified senseRNA and degraded RNA in 45 minutes. The simple protocol consists of just two steps. The 3’end of the RNA is polyA tailed followed by the ligation of biotinylated 3DNA™ dendrimers containing 15 biotins to provide signal amplification. The biotinylated RNA is suitable for multiple downstream applications including profiling microRNA expression levels on the xMAP® platform.

Marligen’s Vantage™ Multiplex Detection Panels are disease-specific microRNA profiling panels formatted on the Luminex xMAP® platform. These fast, simple hybridization assays are ready for detection on the Luminex™ reader within 90 minutes. When coupled with Marligen’s Vantage™ Labeling Kit these assays can detect microRNAs in as little as 100 ng total RNA without the need for enrichment or amplification.

Individual assays in Vantage™ microRNA Multiplex Detection Panels can be configured into Custom-Mixed microRNA Detection Panels. For example, ten microRNAs can be selected from the total list and reconfigured into a single custom-mixed panel. Choose as many assays as you like from the full assay list and send the list to Marligen for a custom quotation. Once you have chosen your specific microRNAs, Marligen scientists will reconfigure the panels to your specifications, manufacture reagents, validate performance, and send you a complete package ready for use.