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Vantage™ microRNA Detection Panels

technical notes faqs

 
 
Marligen’s Vantage™ microRNA Detection Panels offers researchers a fast and simple method for profiling the expression levels of multiple microRNAs from many different sample types including total RNA, enriched low molecular weight (LMW) RNA, and degraded RNA. Download full list of Vantage microRNA Detection Panels.

Advantages:

  • Detects microRNA profiles in all types of RNA sample including enriched or degraded samples*
  • No sample amplification necessary
  • Configuration of assays on xMAP® multiplex bead array allows analysis of multiple microRNAs in one sample
  • Simple hybridization protocol can be completed in 90 minutes
  • Hundreds of samples can be profiled in one day
  • Flexible bead-array format allows disease-specific or customer-specified panels to be quickly put together.

Comparison of Vantage microRNA Detection Panels versus leading technologies for microRNA detection

  Vantage™ microRNA Detection Panels Planar Arrays qRT-PCR
Sample Type      
Total RNA YES* YES# YES
Enriched LMW RNA YES* YES YES
Degraded RNA (RN<8) YES* YES# YES
RT Step NO NO YES
Amplification NO NO YES
Multiplex YES YES NO
<2 hour protocol YES$$ NO YES
Sample Throughput HIGH MEDIUM LOW
Flexible assay format YES NO NO

* Vantage microRNA Detection Panels can detect all RNA sample types. Samples must be labeled with Vantage™ microRNA Labeling Kit prior to assaying on Detection Panels
# Some planar array labeling and detection methods not compatible with total RNA or RNA isolated from FFPE samples.
$$ Time based on Vantage™ microRNA Detection Panel protocol The complete Vantage™ protocol from purification through detection takes < 4 hours.

Vantage™ MicroRNA Detection Panels

Performance Characteristics:

Highly Specific Assays with Minimal Cross-Reactivity Extremely Sensitive Assays with Broad Dynamic Range

 

Oncology Detection Panel 1
Cat. No. 11831-050
50 reactions
Pkg insert

MicroRNA xMAP® Bead # MicroRNA xMAP® Bead # MicroRNA xMAP® Bead #
hsa-let-7a 1 hsa-miR-17 19 hsa-miR-29b-2* 35
hsa-let-7c 2 hsa-miR-181b 20 hsa-miR-29c* 36
hsa-let-7g 3 hsa-miR-185 21 hsa-miR-30d 37
hsa-let-7i 4 hsa-miR-195 22 hsa-miR-34a 38
hsa-miR-100 5 hsa-miR-199a-5p 23 hsa-miR-34b* 39
hsa-miR-106a 6 hsa-miR-200b* 24 hsa-miR-9 40
hsa-miR-10a 7 hsa-miR-205 25 hsa-miR-93 41
hsa-miR-10b 8 hsa-miR-20a 26 hsa-miR-95 42
hsa-miR-125a-5p 9 hsa-miR-21 27 hsa-miR-96 43
hsa-miR-125b 10 hsa-miR-210 28 hsa-miR-99a 44
hsa-miR-132* 13 hsa-miR-212 29 hsa-miR-137 45
hsa-miR-135a 14 hsa-miR-218 30 hsa-miR-182* 46
hsa-miR-136 15 hsa-miR-23b* 31 hsa-miR-221 47
hsa-miR-138 16 hsa-miR-24-2* 32 hsa-miR-372 48
hsa-miR-141* 17 hsa-miR-27a* 33 Control 1 49
hsa-miR-16 18 hsa-miR-29a* 34    

All of the microRNAs detected by the Vantage™ Detection Panels are designated by human sequence nomenclature. Download full list of Vantage microRNA Detection Panels above to obtain homology with other species.

To request a quote or additional information please e-mail customer.service@marligen.com or contact us at 866-464-4990.

Individual assays in Vantage™ microRNA Detection Panels can be configured into custom-mixed Panels. For example, ten microRNAs can be selected from the total list and reconfigured into a single custom-mixed panel. Choose as many assays as you like from the full assay list and send the list to Marligen for a custom quotation. Once you have chosen your specific microRNAs, Marligen scientists will reconfigure the panels to your specifications, manufacture reagents, validate performance, and send you a complete package ready for use.

To request a quote or additional information for custom-mixed Vantage™ microRNA Detection Panels please fill out a quote request. To request a quote or additional information please e-mail customer.service@marligen.com or contact us at 866-464-4990.

All of Marligen Vantage™ Detection Panels are configured to be used in conjunction with the Luminex™ xMAP System for multiplexed analysis. For researchers interested in trying out our assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services. Please enquire if you are interested in microRNA testing services.

 

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