Vantage™ microRNA Detection Panels, microRNA Expression Profiling, microRNA Multiplex Assays

Marligen’s Vantage™ microRNA Detection Panels offers researchers a fast and simple method for profiling the expression levels of multiple microRNAs from many different sample types including total RNA, enriched low molecular weight (LMW) RNA, and degraded RNA. Download full list of Vantage microRNA Detection Panels.

Advantages:

Detects microRNA profiles in all types of RNA sample including enriched or degraded samples*
No sample amplification necessary
Configuration of assays on xMAP® multiplex bead array allows analysis of multiple microRNAs in one sample
Simple hybridization protocol can be completed in 90 minutes
Hundreds of samples can be profiled in one day
Flexible bead-array format allows disease-specific or customer-specified panels to be quickly put together.

Comparison of Vantage microRNA Detection Panels versus leading technologies for microRNA detection

	Vantage™ microRNA Detection Panels	Planar Arrays	qRT-PCR
Sample Type
Total RNA	YES*	YES#	YES
Enriched LMW RNA	YES*	YES	YES
Degraded RNA (RN<8)	YES*	YES#	YES
RT Step	NO	NO	YES
Amplification	NO	NO	YES
Multiplex	YES	YES	NO
<2 hour protocol	YES$$	NO	YES
Sample Throughput	HIGH	MEDIUM	LOW
Flexible assay format	YES	NO	NO

* Vantage microRNA Detection Panels can detect all RNA sample types. Samples must be labeled with Vantage™ microRNA Labeling Kit prior to assaying on Detection Panels
# Some planar array labeling and detection methods not compatible with total RNA or RNA isolated from FFPE samples.
$$ Time based on Vantage™ microRNA Detection Panel protocol The complete Vantage™ protocol from purification through detection takes < 4 hours.

Vantage™ MicroRNA Detection Panels

Performance Characteristics:

Highly Specific Assays with Minimal Cross-Reactivity

No Cross-reactivity Observed Between Unrelated Sequences (print)

Extremely Sensitive Assays with Broad Dynamic Range

Oncology Detection Panel 1
Cat. No. 11831-050
50 reactions
Pkg insert

MicroRNA	xMAP® Bead #	MicroRNA	xMAP® Bead #	MicroRNA	xMAP® Bead #
hsa-let-7a	1	hsa-miR-17	19	hsa-miR-29b-2*	35
hsa-let-7c	2	hsa-miR-181b	20	hsa-miR-29c*	36
hsa-let-7g	3	hsa-miR-185	21	hsa-miR-30d	37
hsa-let-7i	4	hsa-miR-195	22	hsa-miR-34a	38
hsa-miR-100	5	hsa-miR-199a-5p	23	hsa-miR-34b*	39
hsa-miR-106a	6	hsa-miR-200b*	24	hsa-miR-9	40
hsa-miR-10a	7	hsa-miR-205	25	hsa-miR-93	41
hsa-miR-10b	8	hsa-miR-20a	26	hsa-miR-95	42
hsa-miR-125a-5p	9	hsa-miR-21	27	hsa-miR-96	43
hsa-miR-125b	10	hsa-miR-210	28	hsa-miR-99a	44
hsa-miR-132*	13	hsa-miR-212	29	hsa-miR-137	45
hsa-miR-135a	14	hsa-miR-218	30	hsa-miR-182*	46
hsa-miR-136	15	hsa-miR-23b*	31	hsa-miR-221	47
hsa-miR-138	16	hsa-miR-24-2*	32	hsa-miR-372	48
hsa-miR-141*	17	hsa-miR-27a*	33	Control 1	49
hsa-miR-16	18	hsa-miR-29a*	34

All of the microRNAs detected by the Vantage™ Detection Panels are designated by human sequence nomenclature. Download full list of Vantage microRNA Detection Panels above to obtain homology with other species.

To request a quote or additional information please e-mail customer.service@marligen.com or contact us at 866-464-4990.

Individual assays in Vantage™ microRNA Detection Panels can be configured into custom-mixed Panels. For example, ten microRNAs can be selected from the total list and reconfigured into a single custom-mixed panel. Choose as many assays as you like from the full assay list and send the list to Marligen for a custom quotation. Once you have chosen your specific microRNAs, Marligen scientists will reconfigure the panels to your specifications, manufacture reagents, validate performance, and send you a complete package ready for use.

To request a quote or additional information for custom-mixed Vantage™ microRNA Detection Panels please fill out a quote request. To request a quote or additional information please e-mail customer.service@marligen.com or contact us at 866-464-4990.

All of Marligen Vantage™ Detection Panels are configured to be used in conjunction with the Luminex™ xMAP System for multiplexed analysis. For researchers interested in trying out our assays but do not have access to the Luminex instrumentation or who would prefer to outsource the work, our experienced technical staff also performs Testing Services. Please enquire if you are interested in microRNA testing services.

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