Vantage™ microRNA Labeling Kit

Advantages:

• Labeling can be completed in 45 minutes
• Highly sensitive labeling method requires little input RNA (as little as 100g total RNA extracted from tissues)
• LMW RNA enrichment not necessary
• Labels degraded RNA from FFPE samples
• Biotinylated RNA suitable for multiple applications
• Compatible for use with xMAP® microRNA profiling kits

Labeling completed in 45 minutes

This simple protocol consists of just two steps. The 3’end of the RNA is poly(A) tailed followed by the ligation of biotinylated 3DNA™ dendrimers containing 15 biotins to provide signal amplification.

Highly sensitive labeling method requires little input RNA

Marligen’s Vantage™ microRNA labeling and detection kits can detect <1pg (or <250 attomoles) synthetic microRNA. In this example, the limit of detection of miR-210 is 0.09 pg (12 attomoles) of synthetic miR.

Labels degraded RNA

Total RNA was extracted from normal and tumor lung. The total RNA from both samples was determined to be degraded when analyzed using Agilent LabChips®. The RNA samples were labeled and detected using Marligen’s Vantage™ microRNA Labeling Kit and Oncology Detection Panel 1 (Cat. No. 11831-050). A subset of microRNAs detected by the Vantage™ Oncology Panel 1 is shown here. The data demonstrates that expression profiles from degraded RNA can be analyzed using the Marligen Vantage™ labeling and detection method.

Order Vantage™ microRNA Labeling Kit

For international pricing and orders please contact one of our international distributors.

This product or portions thereof is manufactured under license from Genisphere, Inc. Marligen Biosciences, Inc. is the exclusive licensee for the research use of this labeling technology in microRNA Luminex™ bead-based assays. 3DNA™ and Genisphere® are trademarks of Datascope.