Vantage™ Total RNA Purification Kit, Isolation of RNA, Purification of RNA

Marligen’s Vantage Total RNA Purification Kit provides a rapid method for the isolation and purification of high quality total RNA from a variety of different sample types without the use of phenol or chloroform.

Product features:

(1) Spin-column format allows processing of multiple samples in less than 20 minutes

(2) Isolates all RNA species including large (e.g. mRNA) and LMW RNAs (e.g. miRNAs, siRNAs)

Comparison of marligen and qiagen kits]

(3) Excellent yields from a variety of sample types

Total RNA Yields from the Vantage™ Total RNA Purification Kit

Type	Quantity	Yield RNA
Cells
Hepatocytes	1 x 10⁶	30 µg
NIH/3T3 Cells	5x10⁵ cells	4.4 µg (nanodrop)
Serum or plasma	100 µL	200-300 ng
Stem Cells	50,000	2 µg
HeLa	1 x 10⁶	15 µg
MCF-7 cells	1 x 10⁶	10 µg
E. coli	1 x 10⁹	50 ug
S. cerevisiae	1 x 10⁷	30 µg
Tissues
Brain	10 mg	8 µg
Kidney	10 mg	30 µg
Liver	10 mg	30 µg
Lung	10 mg	10 µg
Spleen	10 mg	35 µg

(4) Comparison of RT-PCR products generated from total RNA purified using Marligen’s kit, a leading competitor's kits and the Trizol extraction method.

4 RT-PCR experiment with range of RNA sizes

(5) Total RNA can be isolated from small amounts of sample – even from a single cell.

Total RNA purified from a single cell can be analyzed by qRT-PCR

(6) Total RNA can be purified from a diverse array of sample types including cultured cells, tissues, blood, bacteria, yeast, fungi, plants, and nasal or throat swabs.

Total RNA isolated from a variety of different plant tissues

Total RNA isolated from yeast

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